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pcre ires puror  (Addgene inc)


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  • 93

    Structured Review

    Addgene inc pcre ires puror
    Pcre Ires Puror, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 37 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcre+ires+puror/Cre-IRES-PuroR+(Plasmid+%2330205)/pm31884373-46-26-32
    Average 93 stars, based on 37 article reviews
    pcre ires puror - by Bioz Stars, 2026-10
    93/100 stars

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    Transfection:

    Article Title: Generation of a SOX9-tdTomato reporter human iPSC line, MCRIi001-A-2, using CRISPR/Cas9 editing.
    Article Snippet: .. To excise the puromycin resistance gene, ~1 × 106 cells of a successfully targeted heterozygous clone were transfected with 3 μg of the Cre recombinase-expressing plasmid, pCre-IRES-PuroR (a gift from Darrell Kotton, Addgene plasmid # 30,205), and 0.2 μg pEF-BOS-GFP as above. .. On the third day, GFPpositive single cells were flow sorted (FACSAria Fusion Cell Sorter, BD Biosciences) into 96 well plates plated with MEFs.

    Plasmid Preparation:

    Article Title: Generation of a SOX9-tdTomato reporter human iPSC line, MCRIi001-A-2, using CRISPR/Cas9 editing.
    Article Snippet: .. To excise the puromycin resistance gene, ~1 × 106 cells of a successfully targeted heterozygous clone were transfected with 3 μg of the Cre recombinase-expressing plasmid, pCre-IRES-PuroR (a gift from Darrell Kotton, Addgene plasmid # 30,205), and 0.2 μg pEF-BOS-GFP as above. .. On the third day, GFPpositive single cells were flow sorted (FACSAria Fusion Cell Sorter, BD Biosciences) into 96 well plates plated with MEFs.



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    93
    Addgene inc pcre ires puror
    Pcre Ires Puror, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcre+ires+puror/Cre-IRES-PuroR+(Plasmid+%2330205)/pm31884373-46-26-32
    Average 93 stars, based on 1 article reviews
    pcre ires puror - by Bioz Stars, 2026-10
    93/100 stars
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